Phospho nfat flow antibody
WebNFAT 1 (also known as NFATc2 or NFATp) is a 154 kDa member of this family that is regulated by the calcium-dependent phosphatase calcineurin. When calcineurin is … WebFor Analyzing Cell Signaling Pathway Activation. Phosphorylation, the addition of a phosphate group to serine, threonine or tyrosine residues, is an important cellular process utilized to send cellular signals from the membrane to the nucleus. For example, changes in physiological conditions may require immediate changes in gene expression.
Phospho nfat flow antibody
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WebMay 14, 2024 · For both NFAT-mediated GFP ... Cell pellets were resuspended in the residual media by pulsed vortexing. When required, primary antibodies at ~10 µg/ml, diluted in flow wash buffer (100 µl ... The resuspended pellet was then incubated with the phospho-ERK1/2 (Thr 202 /Tyr 204) antibody directly conjugated with Alexa Fluor 488 for 45 min at ... WebThe costimulatory receptor, CD28, synergizes with the T cell antigen receptor (TCR) to promote IL-2 production, cell survival and proliferation. Despite their profound synergy, the obligatory interdependence of the signaling pathways initiated by
WebProduct Specific Information Phospho-NFATC1 (Ser172) Polyclonal Antibody detects endogenous levels of NFATC1 only when … WebThis antibody conjugate is suitable for intracellular staining of human cell lines and peripheral blood mononuclear cells using BD Cytofix™ Fixation Buffer. Any of the three BD Phosflow™ permeabilization buffers may be used. Product Notices This reagent has been pre-diluted for use at the recommended Volume per Test.
WebFour NFAT proteins, encoded on separate genes and expressed as several splice variants, have been described: NFAT1 (also known as NFATp or NFATc2), NFAT2 (NFATc or … WebHuman Phospho-NFATC1 (S172) Antibody Summary. Detects human NFATC1 in direct ELISAs. Phosphopeptide containing the human S172 …
WebThis antibody detects both forms NFAT1 - a ~140 kDa protein representing phosphorylated NFAT1 in resting immune cells, and a ~120 kDa protein in stimulated cells that represents …
WebName. Anti-NFAT2 (Phospho-Ser172) Antibody. Description. Rabbit Polycolonal antibody to NFAT2 (Phospho-Ser172) Specificity. NFAT2 (Phospho-Ser172) Antibody detects endogenous levels of NFAT2 only … christmas golf clipartWebPhospho-specific antibodies allow for the phosphorylation state of individual protein residues to be determined by standard, immunoblotting techniques.26 Several phospho-specific antibodies have been developed for Raf-1, including antibodies that recognize phospho-Ser-338 and phospho-Tyr-340/341. christmas golf cart themesWebDec 26, 2024 · Phosphate is one of the most important molecular elements to normal cellular functions within the body. It acts as an integral component of nucleic acids and is used to replicate DNA and RNA. It is an … christmas golf clip art freeWebWhen selecting a phospho-specific antibody for your experiments, it is crucial to ensure that the antibody specifically detects the protein of interest only when it is phosphorylated at the indicated site. It is also essential to check that the antibody detects a band at the correct molecular weight for your phosphorylated target protein. christmas golf jumpers for menWebAnti-phospho-NFAT1 (pSer54) antibody produced in rabbit (affinity isolated antibody, buffered aqueous solution); Suitable for western blot; Rabbit polyclonal anti-phospho-NFAT1 (pSer54) antibody is used to tag phospho-NFAT1 (pSer54) for detection and quantitation by immunocytochemical and USEN ApplicationsProductsServicesSupport N8536 All Photos(1) gesthotel ferraraWebThis antibody conjugate is suitable for intracellular staining of human whole blood (using BD Phosflow™ Lyse/Fix Buffer) and peripheral blood mononuclear cells (using BD Cytofix™ Fixation Buffer or BD Phosflow™ Fix Buffer I). christmas golf jokesWebNFAT proteins are predominantly expressed in cells of the immune system, but are also expressed in skeletal muscle, keratinocytes, and adipocytes, regulating cell differentiation … christmas golf cart decoration ideas