NettetITS is a mixture of insulin, transferrin, and selenium. It is a general cell supplement designed for use in classical media such as DMEM, RPMI-1640 and nutrient media such as Ham’s F-12, DMEM/F-12. ITS enhances cell proliferation and decreases the serum requirement of many cell types [1, 2, 3]. When ITS is used with a low percent serum ... Nettet11. apr. 2024 · Briefly, 2 × 10 5 hESCs were cultured as a suspension in hESC medium that was conditioned on human foreskin fibroblasts and contained DMEM/F12, 20% knockout serum replacement (KoSR, catalog no. 10828028, Gibco, USA), 2 mM L-glutamine (catalog no. 35050-038, Gibco, USA), 1 mM nonessential amino acids …
Insulin, transferrin, selenium solution Sigma-Aldrich
Nettet12. sep. 2024 · Mix with 10% MEM, 1% antibiotic/antimycotic and 1% insulin-transferrin-selenium. For example, for 100 mL of transfection medium, use 88 mL of salt-glucose-glycine solution, 10 mL of MEM and 1 mL each of antibiotic/antimycotic and insulin-transferrin-selenium solution. 4. Sterile filter the medium using a filter with 0.22 μm … Nettet14. apr. 2024 · After centrifugation, tumour cells were harvested and cultured in AR-5 medium (5% FBS, 1× GlutaMAX (Thermo Fisher Scientific), 1× ITS (Insulin-Transferrin-Selenium, Thermo Fisher Scientific), 1% ... how to order a new nedbank card
The amount of membrane cholesterol required for robust cell
NettetInsulin-Transferrin-Selenium Supplement (100X) 41400-045 . Insulin-Transferrin-Selenium-A Supplement (100X) 51300-044 . 10 mL : 2°C to 8°C . 18 months : Insulin-Transferrin-Selenium-X Supplement (100X) 51500-056 * Shelf life duration is determined from Date of Manufacture. Product use . For Research Use Only. Not for use in … NettetInsulin-Transferrin-Sodium Selenite Supplement suitable for cell culture, lyophilized, pkg of 50 mg ... Selenium is very often necessary for optimal cell growth. ... sodium … NettetITS-X solution is routinely used, in combination with 2 to 4% FBS, to support cell types that require ethanolamine, such as bronchial epithelial cell. Insulin promotes glucose and amino acid uptake, lipogenesis, intracellular transport, and the synthesis of proteins and nucleic acids. Transferrin is an iron carrier and may also help to reduce ... mvs pcauth