WebApr 12, 2024 · In X-ray crystallography and cryo-EM, experimental maps can be heterogeneous, showing different regions of the structure with different level of details. In this work we interpret the heterogeneity in terms of two parameters, assigned individually for each atom, combining the conventional parameter of atomic displacement with the … WebScipion is an image processing framework for obtaining 3D models of macromolecular complexes using Electron Microscopy (3DEM). It integrates several software …
Munan Shaik - Protein Engineering at Modex …
WebAug 4, 2024 · Cryo-EM analysis of a membrane protein embedded in the liposome Membrane proteins (MPs) used to be the most difficult targets for structural biology when … WebApr 11, 2024 · Data analysis and interpretation and preparation: submitting of manuscripts for publication (40%) Grant Proposals: assisting in preparing grant applications ... , including proficiency in troubleshooting and optimization of sample/grid conditions for cryoEM analysis and data collection and processing. Desire to think independently across ... gustman used cars for sale
From structure to sequence: Antibody discovery using cryoEM
Web1 day ago · In this pre-PS conformation, the linker is in a 90°-bent conformation, which is similar to the single-particle cryo-EM analysis of cytoplasmic and axonemal dyneins heads in the presence of ADP-VO 4 (Roberts et al, 2009; Schmidt et al, 2015), while the stalks are visible and point proximally toward the adjacent B-tubule (green and yellow in Fig ... WebThus, efficient data acquisition and on-the-fly analysis of a large number of dose-fractionated image stacks become a serious challenge to any cryoEM data acquisition system. We have developed a computer-assisted system, named UCSFImage4, for semi-automated cryo-EM image acquisition that implements an asynchronous data acquisition … WebNov 25, 2024 · We present the first cryoEM structure of a protein in CyclAPols, at 3.2 Å resolution, demonstrating their applicability to high-resolution structure determination by … gust meaning spanish